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KMID : 0811720120160030205
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Korean Journal of Physiology & Pharmacology
2012 Volume.16 No. 3 p.205 ~ p.209
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Development of Estimation Methods of Skin Oxidation and Evaluation of Anti-Oxidative Effects of Genistein in Topical Formulations
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Kim Seong-Yeon
Na Yeon-Joo
Kim Dong-ju
Kim Yeong-seok
Kim Hyeong-Min
Hwang Sung-Ha
Kwak Ji-Yeon
Kuh Hyo-Jeong
Lee Jae-Hwi
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Abstract
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The objective of the present study was to establish the method of measurement of hydrogen peroxide and to estimate the anti-oxidative effect of genistein in the skin. UVB induced skin oxidation and anti-oxidative effect of genistein formulations were evaluated by determining levels of hydrogen peroxide. The mechanism involved in the determination of hydrogen peroxide is based on a color reaction between ferric ion (Fe3＋) and xylenol orange, often called FOX assay and subsequent monitoring of absorbance values of the reactant at 540 nm. The reaction was to some extent pH-dependent and detection sensitivity was greatest at pH 1.75. Genistein liposomal gel demonstrated better anti-oxidative effect with regard to lowering hydrogen peroxide levels elevated by UVB irradiation compared to genistein-suspended gel. A linear relationship has been observed between anti-oxidative effect of genistein and drug deposition in the skin tissue. Genistein liposomal gel resulting in the localization of the drug in the deeper skin led to improved anti-oxidative effect compared to genistein gel. The suggested method for evaluation of oxidation of the skin can be used as a tool to screen effective anti-oxidative agents and their delivery systems acting on the skin.
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KEYWORD
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Oxidation, Skin, Hydrogen peroxide, Genistein
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